Complete Genome Sequence of the First KPC-Type Carbapenemase-Positive Proteus mirabilis Strain from a Bloodstream Infection

Vincenzo Di Pilato; Adriana Chiarelli; Christine J Boinett; Eleonora Riccobono; Simon R Harris; Marco Maria D'Andrea; Nicholas R Thomson; Gian Maria Rossolini; Tommaso Giani

doi:10.1128/genomeA.00607-16

Complete Genome Sequence of the First KPC-Type Carbapenemase-Positive Proteus mirabilis Strain from a Bloodstream Infection

Genome Announc. 2016 Jun 23;4(3):e00607-16. doi: 10.1128/genomeA.00607-16.

Authors

Vincenzo Di Pilato¹, Adriana Chiarelli², Christine J Boinett³, Eleonora Riccobono⁴, Simon R Harris³, Marco Maria D'Andrea², Nicholas R Thomson³, Gian Maria Rossolini⁵, Tommaso Giani⁶

Affiliations

¹ Department of Surgery and Translational Medicine, University of Florence, Florence, Italy.
² Department of Medical Biotechnologies, University of Siena, Siena, Italy.
³ Pathogen Genomics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
⁴ Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
⁵ Department of Medical Biotechnologies, University of Siena, Siena, Italy Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy Don Carlo Gnocchi Foundation, Florence, Italy.
⁶ Department of Medical Biotechnologies, University of Siena, Siena, Italy tommaso.giani@unisi.it.

Abstract

Sequencing of the blaKPC-positive strain Proteus mirabilis AOUC-001 was performed using both the MiSeq and PacBio RS II platforms and yielded a single molecule of 4,272,433 bp, representing the complete chromosome. Genome analysis showed the presence of several acquired resistance determinants, including two copies of blaKPC-2 carried on a fragment of a KPC-producing plasmid previously described in Klebsiella pneumoniae.

Abstract

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