Complete Genome Sequences of Five Bluetongue Virus (BTV) Vaccine Strains from a Commercial Live Attenuated Vaccine, a BTV-4 Field Strain from South Africa, and a Reassortant Strain Isolated from Experimentally Vaccinated Cattle

Carien van den Bergh; Peter Coetzee; Alan J Guthrie; Misha le Grange; Estelle H Venter

doi:10.1128/genomeA.00462-16

Complete Genome Sequences of Five Bluetongue Virus (BTV) Vaccine Strains from a Commercial Live Attenuated Vaccine, a BTV-4 Field Strain from South Africa, and a Reassortant Strain Isolated from Experimentally Vaccinated Cattle

Genome Announc. 2016 Jun 23;4(3):e00462-16. doi: 10.1128/genomeA.00462-16.

Authors

Carien van den Bergh¹, Peter Coetzee², Alan J Guthrie², Misha le Grange², Estelle H Venter²

Affiliations

¹ Department of Veterinary Tropical Diseases, Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa cvdbprivate@fastmail.fm.
² Department of Veterinary Tropical Diseases, Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.

Abstract

This is a report of the complete genome sequences of plaque-selected isolates of each of the five virus strains included in a South African commercial trivalent bluetongue virus (BTV) attenuated live virus vaccine, a BTV-4 field strain isolated from Rustenburg, South Africa, in 2011, and a bluetongue reassortant (bluetongue virus 4 strain 4/O. aries-tc/ZAF/11/OBP-115) isolated from experimentally vaccinated cattle. Full-genome sequencing and phylogenetic analyses show that the bluetongue virus 9 strain 9/B. taurus-tc/ZAF/15/Onderstepoort_B02b is a reassortant virus containing segments from both BTV-9 and BTV-8.