Absolute nutrient concentration measurements in cell culture media: (1)H q-NMR spectra and data to compare the efficiency of pH-controlled protein precipitation versus CPMG or post-processing filtering approaches

Luca Goldoni; Tiziana Beringhelli; Walter Rocchia; Natalia Realini; Daniele Piomelli

doi:10.1016/j.dib.2016.05.054

Absolute nutrient concentration measurements in cell culture media: (1)H q-NMR spectra and data to compare the efficiency of pH-controlled protein precipitation versus CPMG or post-processing filtering approaches

Data Brief. 2016 May 30:8:387-93. doi: 10.1016/j.dib.2016.05.054. eCollection 2016 Sep.

Authors

Luca Goldoni¹, Tiziana Beringhelli², Walter Rocchia³, Natalia Realini¹, Daniele Piomelli⁴

Affiliations

¹ Drug Discovery and Development, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy.
² Department of Chemistry, University of Milan, via Golgi 19, 20133 Milano, Italy.
³ CONCEPT Lab, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy.
⁴ Drug Discovery and Development, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy; Departments of Anatomy and Neurobiology, Pharmacology and Biological Chemistry, University of California, Irvine, CA 92697, USA.

Abstract

The NMR spectra and data reported in this article refer to the research article titled "A simple and accurate protocol for absolute polar metabolite quantification in cell cultures using q-NMR" [1]. We provide the (1)H q-NMR spectra of cell culture media (DMEM) after removal of serum proteins, which show the different efficiency of various precipitating solvents, the solvent/DMEM ratios, and pH of the solution. We compare the data of the absolute nutrient concentrations, measured by PULCON external standard method, before and after precipitation of serum proteins and those obtained using CPMG (Carr-Purcell-Meiboom-Gill) sequence or applying post-processing filtering algorithms to remove, from the (1)H q-NMR spectra, the proteins signal contribution. For each of these approaches, the percent error in the absolute value of every measurement for all the nutrients is also plotted as accuracy assessment.

Keywords: 1H NMR; Accuracy; CPMG; Deconvolution; PULCON; pH-controlled serum removal.