Cell line donor genotype and its influence on experimental phenotype: Toll-like receptor SNPs and potential variability in innate immunity

Sara A Tokarz; Jessica DeValk; Wenxiang Luo; Bikash R Pattnaik; Steven J Schrodi; De-Ann M Pillers

doi:10.1016/j.ymgme.2016.04.011

Cell line donor genotype and its influence on experimental phenotype: Toll-like receptor SNPs and potential variability in innate immunity

Mol Genet Metab. 2016 Jul;118(3):147-152. doi: 10.1016/j.ymgme.2016.04.011. Epub 2016 Apr 22.

Authors

Sara A Tokarz¹, Jessica DeValk¹, Wenxiang Luo¹, Bikash R Pattnaik², Steven J Schrodi³, De-Ann M Pillers⁴

Affiliations

¹ Division of Neonatology & Newborn Nursery, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Pediatrics, University of Wisconsin-Madison, Madison, WI 53706, USA.
² Division of Neonatology & Newborn Nursery, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Pediatrics, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, USA; McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI 53706, USA.
³ Computation and Informatics in Biology and Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA; Center for Human Genetics, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA.
⁴ Division of Neonatology & Newborn Nursery, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Pediatrics, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Medical Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA; McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI 53706, USA; J.F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, Madison, WI 53706, USA. Electronic address: pillers@wisc.edu.

PMID: 27324283
DOI: 10.1016/j.ymgme.2016.04.011

Abstract

Cell lines are used to model a disease and provide valuable information regarding phenotype, mechanism, and response to novel therapies. Derived from individuals of diverse genetic backgrounds, the cell's genetic complement predicts the phenotype, and although some lines have been sequenced, little emphasis has been placed on genotyping. Toll-like receptors (TLRs) are essential in initiating the inflammatory cascade in response to infection. TLR single nucleotide polymorphism (SNP) alleles may predict an altered innate immune response: a SNP can affect TLR-dependent pathways and may alter experimental results. Thus, genotype variation may have far-reaching implications when using cell lines to model phenotypes. We recommend that cell lines be genotyped and cataloged in a fashion similar to that used for bacteria, with cumulative information being archived in an accessible central database to facilitate the understanding of SNP cell phenotypes reported in the literature.

Keywords: Catalog; Human; SNP; Variome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Genotype
Humans
Immunity, Innate*
Models, Biological
Phenotype
Polymorphism, Single Nucleotide*
Signal Transduction
Toll-Like Receptors / genetics*

Substances

Toll-Like Receptors