Structure of Malformin A, a Phytotoxic Metabolite Produced by Aspergillus niger

K W Kim; F Sugawara; S Yoshida; N Murofushi; N Takahashi; R W Curtis

doi:10.1271/bbb.57.240

Structure of Malformin A, a Phytotoxic Metabolite Produced by Aspergillus niger

Biosci Biotechnol Biochem. 1993 Jan;57(2):240-3. doi: 10.1271/bbb.57.240.

Authors

K W Kim^{1

2}, F Sugawara¹, S Yoshida¹, N Murofushi^{1

2}, N Takahashi¹, R W Curtis^{1

3}

Affiliations

¹ a The Institute of Physical and Chemical Research (RIKEN) , Wako , Saitama 351-01 , Japan.
² b Department of Agricultural Chemistry , The University of Tokyo , Bunkyo-ku , Tokyo 113 , Japan.
³ c Department of Botany and Plant Pathology , Purdue University , West Lafayette , IN 49707 , U.S.A.

PMID: 27314776
DOI: 10.1271/bbb.57.240

Abstract

Malformins-produced by Aspergillus niger were separated by HPLC and subjected to structural determination. Amino acid analyses and mass spectra suggested that all of them structurally resembled cyclic pentapeptide malformin A1. Two-dimensional NMR experiments and MS/MS experiments led us to deduce cyclo-D-cysteinyl-D-cysteinyl-L-amino acid-D-amino acid-L-amino acid as being the essential structure of malformins.