Flow cytometric enumeration of bacterial in the coral surface mucus layer

Yvan Bettarel; Mai Chi Thanh; Got Patrice; Adingra Antoinette; Kouadio-Ngbesso Nadège; Van Ngoc Bui; Bouvier Thierry

doi:10.1016/j.mimet.2016.05.032

Flow cytometric enumeration of bacterial in the coral surface mucus layer

J Microbiol Methods. 2016 Sep:128:16-19. doi: 10.1016/j.mimet.2016.05.032. Epub 2016 Jun 11.

Authors

Yvan Bettarel¹, Mai Chi Thanh², Got Patrice¹, Adingra Antoinette³, Kouadio-Ngbesso Nadège³, Van Ngoc Bui², Bouvier Thierry¹

Affiliations

¹ UMR MARBEC (IRD, CNRS, UM, IFREMER) Marine Biodiversity, Exploitation and Conservation, Montpellier, France.
² Institute of Biotechnology of Hanoi, VAST, Viet Nam.
³ Centre de Recherche Océanologique d'Abidjan, Cote d'Ivoire.

PMID: 27302040
DOI: 10.1016/j.mimet.2016.05.032

Abstract

The direct counts of bacteria inhabiting coral mucus were performed by flow cytometry testing four fluorescent dyes (SYBR®Green I, HCS, TOPRO®3, SYTO®62) with three different scleractinian species. Results obtained with SYTO62 were the most reliable based on the comparison with standardized epifluorescence counts and the resolution of cytograms.

Keywords: Bacteria; Corals; Epibionts; Fluorescent dyes; Microscopy; Mucus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anthozoa / microbiology*
Bacteria / isolation & purification*
Colony Count, Microbial*
Flow Cytometry*
Fluorescent Dyes
Microbial Viability

Substances

Fluorescent Dyes