Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells

Juntao Tan; Nuo Yang; Zixi Hu; Jing Su; Jianhong Zhong; Yang Yang; Yating Yu; Jianmeng Zhu; Dabin Xue; Yingying Huang; Zongqiang Lai; Yong Huang; Xiaoling Lu; Yongxiang Zhao

doi:10.1186/s11671-016-1512-8

Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells

Nanoscale Res Lett. 2016 Dec;11(1):298. doi: 10.1186/s11671-016-1512-8. Epub 2016 Jun 14.

Authors

Juntao Tan¹, Nuo Yang¹, Zixi Hu¹, Jing Su¹, Jianhong Zhong², Yang Yang³, Yating Yu¹, Jianmeng Zhu¹, Dabin Xue¹, Yingying Huang¹, Zongqiang Lai¹, Yong Huang^{4

5}, Xiaoling Lu⁶, Yongxiang Zhao⁷

Affiliations

¹ National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi, 530021, China.
² Department of Oncologic Surgery, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, China.
³ Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
⁴ National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi, 530021, China. huangyong503@126.com.
⁵ Department of Thoracic Surgery, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, China. huangyong503@126.com.
⁶ National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi, 530021, China. luwuliu@163.com.
⁷ National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi, 530021, China. yongxiang_zhao@126.com.

Abstract

A simple, highly sensitive method to detect leukemia cells has been developed based on aptamer-modified fluorescent silica nanoparticles (FSNPs). In this strategy, the amine-labeled Sgc8 aptamer was conjugated to carboxyl-modified FSNPs via amide coupling between amino and carboxyl groups. Sensitivity and specificity of Sgc8-FSNPs were assessed using flow cytometry and fluorescence microscopy. These results showed that Sgc8-FSNPs detected leukemia cells with high sensitivity and specificity. Aptamer-modified FSNPs hold promise for sensitive and specific detection of leukemia cells. Changing the aptamer may allow the FSNPs to detect other types of cancer cells.