A novel chemiluminescent immunoassay for detection of Toxoplasma gondii IgG in human sera

Lucyna Holec-Gąsior; Bartłomiej Ferra; Justyna Czechowska; Illia E Serdiuk; Karol Krzymiński; Józef Kur

doi:10.1016/j.diagmicrobio.2016.05.013

A novel chemiluminescent immunoassay for detection of Toxoplasma gondii IgG in human sera

Diagn Microbiol Infect Dis. 2016 Aug;85(4):422-5. doi: 10.1016/j.diagmicrobio.2016.05.013. Epub 2016 May 24.

Authors

Lucyna Holec-Gąsior¹, Bartłomiej Ferra², Justyna Czechowska³, Illia E Serdiuk³, Karol Krzymiński³, Józef Kur²

Affiliations

¹ Gdańsk University of Technology, Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, 11/12 G. Narutowicza Str., 80-233, Gdańsk, Poland. Electronic address: holec@pg.gda.pl.
² Gdańsk University of Technology, Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, 11/12 G. Narutowicza Str., 80-233, Gdańsk, Poland.
³ University of Gdańsk, Faculty of Chemistry, Department of Physical Chemistry, 63 Wita Stwosza Str., 80-308, Gdańsk, Poland.

PMID: 27298047
DOI: 10.1016/j.diagmicrobio.2016.05.013

Abstract

This study describes Toxoplasma gondii IgG chemiluminescent immunoassay (CLIA) based on the use of a novel immunochemical reagents in the form of the conjugates of original acridinium ester (AE) labels attached to antibodies and SAG2-GRA1-ROP1L chimeric antigen and shows that this test is useful for diagnostic purposes.

Keywords: Acridinium esters; CLIA; Chemiluminescence; Serological detection.

Publication types

Evaluation Study

MeSH terms

Antibodies, Protozoan / blood*
Diagnostic Tests, Routine / methods*
Humans
Immunoassay / methods*
Immunoglobulin G / blood*
Luminescent Measurements
Toxoplasma / immunology*
Toxoplasmosis / diagnosis*

Substances

Antibodies, Protozoan
Immunoglobulin G