MALDI-TOF MS is more accurate than VITEK II ANC card and API Rapid ID 32 A system for the identification of Clostridium species

Young Jin Kim; Si Hyun Kim; Hyun-Jung Park; Hae-Geun Park; Dongchul Park; Sae Am Song; Hee Joo Lee; Dongeun Yong; Jun Yong Choi; Joong-Ki Kook; Hye Ran Kim; Jeong Hwan Shin

doi:10.1016/j.anaerobe.2016.06.004

MALDI-TOF MS is more accurate than VITEK II ANC card and API Rapid ID 32 A system for the identification of Clostridium species

Anaerobe. 2016 Aug:40:73-5. doi: 10.1016/j.anaerobe.2016.06.004. Epub 2016 Jun 11.

Authors

Affiliations

¹ Department of Laboratory Medicine, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
² Department of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of Korea; Paik Institute for Clinical Research, Inje University College of Medicine, Busan, Republic of Korea.
³ Department of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of Korea.
⁴ Department of Laboratory Medicine and Research Institute of Antimicrobial Resistance, Yonsei University College of Medicine, Seoul, Republic of Korea.
⁵ Division of Infectious Disease, Department of Internal Medicine and AIDS Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea.
⁶ Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.
⁷ Department of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of Korea; Paik Institute for Clinical Research, Inje University College of Medicine, Busan, Republic of Korea. Electronic address: jhsmile@inje.ac.kr.

PMID: 27296834
DOI: 10.1016/j.anaerobe.2016.06.004

Abstract

All 50 Clostridium difficile strains were definitely identified by Vitek2 system, Rapid ID 32A system, and MALDI-TOF. For 18 non-difficile Clostridium strains, the identification results were correct in 2, 0, and 17 strains by Vitek2, Rapid ID 32A, and MALDI-TOF, respectively [corrected].

Keywords: Clostridium; MALDI-TOF MS; Mass spectrometry.

MeSH terms

Clostridioides difficile / genetics
Clostridioides difficile / isolation & purification*
Enterocolitis, Pseudomembranous / diagnosis*
Enterocolitis, Pseudomembranous / microbiology
Feces / microbiology
Humans
RNA, Ribosomal, 16S / genetics*
Sensitivity and Specificity
Sequence Analysis, RNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

RNA, Ribosomal, 16S