Light-sheet imaging of mammalian development

Gustavo de Medeiros; Bálint Balázs; Lars Hufnagel

doi:10.1016/j.semcdb.2015.11.001

Light-sheet imaging of mammalian development

Semin Cell Dev Biol. 2016 Jul:55:148-55. doi: 10.1016/j.semcdb.2015.11.001.

Authors

Gustavo de Medeiros¹, Bálint Balázs¹, Lars Hufnagel²

Affiliations

¹ Cell Biology and Biophysics Unit, EMBL Heidelberg, Germany.
² Cell Biology and Biophysics Unit, EMBL Heidelberg, Germany. Electronic address: lars.hufnagel@embl.de.

PMID: 27288888
DOI: 10.1016/j.semcdb.2015.11.001

Abstract

Tackling modern cell and developmental biology questions requires fast 3D imaging with sub-cellular resolution over extended periods of time. Fluorescence microscopy has emerged as a powerful tool to image biological samples with high spatial and temporal resolution with molecular specificity. In particular, the highly efficient illumination and detection scheme of light-sheet fluorescence microscopy is starting to revolutionize the way we can monitor cellular and developmental processes in vivo. Here we summarize the state-of-the art of light-sheet imaging with a focus on mammalian development - from instrumentation, mounting and sample handling to data processing.

Keywords: Light-sheet; Live-imaging; Microscopy; Mouse embryo.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Aging / physiology
Animals
Embryonic Development
Imaging, Three-Dimensional
Mammals / embryology*
Microscopy, Fluorescence / methods*