Effects of different concentrations of metal ions on degradation of adenosine triphosphate in common carp (Cyprinus carpio) fillets stored at 4°C: An in vivo study

Abstract

The impact of different concentrations of Na(+), K(+), Ca(2+), Mg(2+), Fe(2+), and Zn(2+) on the degradation of adenosine triphosphate (ATP) and the influence of these ions on the activity of adenosine monophosphate deaminase (AMP-deaminase) and acid phosphatase (ACP) in common carp fillets (in vivo) during 4°C storage was examined. The content of ATP, inosine monophosphate (IMP), and hypoxanthine (Hx), and the activity of AMP-deaminase and ACP were determined. Results indicated that the effects of different concentrations of six kinds of metal ions on AMP-deaminase and ACP were not the same. Na(+), K(+), Fe(2+), and Zn(2+) enhanced AMP-deaminase activity, which led to the rapid degradation of ATP and to the generation of a large quantity of IMP within a short time. Ca(2+) and Mg(2+) delayed the change in AMP-deaminase and ACP activity in carp and caused a further delay in the degradation of ATP. Fe(2+) and Zn(2+) inhibited ACP activity, which reduced the decomposition of IMP and the formation of Hx.

Keywords: ACP; AMP-deaminase; ATP degradation; Adenosine triphosphate (PubChem CID: 5957); Calcium ion (PubChem CID: 271); Common carp; Ferrous ion (PubChem CID: 27284); Hypoxanthine (PubChem CID: 790); Inosine mono-phosphate (PubChem CID: 8582); Magnesium ion (PubChem CID: 888); Metal ions; Potassium ion (PubChem CID: 813); Sodium ion (PubChem CID: 923); Zinc ion (PubChem CID: 32051).