Recently we have explored and developed approaches imaging using confocal/two-photon microscopy, which enables simultaneous high-resolution assessment of specifically fluorescently marked cells in conjunction with structural components of the tissues visualized via harmonic generated signals. This approach uses commercially available confocal and two-photon laser microscope and automated user-interactive image analysis methods based on commercially available software packages allowing easy implementation in usual microscopy facilities.
Keywords: Adipose tissue; Cell tracking; Fluorescent proteins; Lymph node; Multiphoton microscopy; Second harmonic generation; Third harmonic generation.