Enhancement of Lipid Production of Chlorella Pyrenoidosa Cultivated in Municipal Wastewater by Magnetic Treatment

Appl Biochem Biotechnol. 2016 Nov;180(6):1043-1055. doi: 10.1007/s12010-016-2151-3. Epub 2016 Jun 4.

Abstract

Despite the significant breakthroughs in research on microalgae as a feedstock for biodiesel, its production cost is still much higher than that of fossil diesel. One possible solution to overcome this problem is to optimize algal growth and lipid production in wastewater. The present study examines the feasibility of using magnetic treatment for enhancement of algal lipid production and wastewater treatment in outdoor-cultivated Chlorella pyrenoidosa. Results confirmed that magnetic treatment significantly enhances biomass and lipid productivity of C. pyrenoidosa by 12 and 10 %, respectively. Application of magnetic field in a semi-continuous culture resulted in highly treated wastewater with total nitrogen maintained under 15 mg L-1, ammonia nitrogen below 5 mg L-1, total phosphorus less than 0.5 mg L-1, and CODCr less than 50 mg L-1. In addition, magnetic treatment resulted in a decrease of wastewater turbidity, an increase of bacterial numbers, and an increase of active oxygen in wastewater which might be attributed to the enhancement of growth and lipid production of C. pyrenoidosa.

Keywords: Biodiesel; Chlorella pyrenoidosa; Lipid enhancement; Magnetic treatment; Wastewater treatment.

MeSH terms

  • Ammonium Compounds / analysis
  • Batch Cell Culture Techniques
  • Biological Oxygen Demand Analysis
  • Biomass
  • Chlorella / growth & development*
  • Chlorella / metabolism*
  • Chlorella / radiation effects
  • Chlorophyll / analysis
  • Chlorophyll A
  • Cities*
  • Colony Count, Microbial
  • Hydrogen-Ion Concentration
  • Light
  • Lipids / biosynthesis*
  • Magnetic Fields
  • Magnetics / methods*
  • Nephelometry and Turbidimetry
  • Nitrogen / analysis
  • Phosphorus / analysis
  • Spectroscopy, Fourier Transform Infrared
  • Temperature
  • Wastewater / chemistry*

Substances

  • Ammonium Compounds
  • Lipids
  • Waste Water
  • Chlorophyll
  • Phosphorus
  • Nitrogen
  • Chlorophyll A