Detection of the plasmid-mediated colistin-resistance gene mcr-1 in clinical isolates and stool specimens obtained from hospitalized patients using a newly developed real-time PCR assay

J Antimicrob Chemother. 2016 Aug;71(8):2344-6. doi: 10.1093/jac/dkw192. Epub 2016 Jun 3.

Authors

R H T Nijhuis¹, K T Veldman², J Schelfaut³, A Van Essen-Zandbergen², E Wessels³, E C J Claas³, J Gooskens³

Affiliations

¹ Department of Medical Microbiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands r.h.t.nijhuis@lumc.nl.
² Department of Bacteriology and Epidemiology, Central Veterinary Institute (CVI) of Wageningen UR, PO Box 65, 8200 AB Lelystad, The Netherlands.
³ Department of Medical Microbiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.

PMID: 27261263
DOI: 10.1093/jac/dkw192

No abstract available

Publication types

Letter

MeSH terms

Colistin / pharmacology*
Drug Resistance, Bacterial / genetics*
Escherichia coli / genetics
Escherichia coli / isolation & purification
Escherichia coli Infections / epidemiology
Escherichia coli Infections / microbiology
Escherichia coli Proteins / genetics*
Feces / microbiology
Genes, Bacterial
Hospitalization
Humans
Multilocus Sequence Typing
Netherlands / epidemiology
Plasmids / genetics*
Real-Time Polymerase Chain Reaction / methods*
Tertiary Care Centers

Substances

Escherichia coli Proteins
MCR-1 protein, E coli
Colistin