Extraction of DNA from Skeletal Remains

Suni M Edson; Timothy P McMahon

doi:10.1007/978-1-4939-3597-0_6

Extraction of DNA from Skeletal Remains

Methods Mol Biol. 2016:1420:69-87. doi: 10.1007/978-1-4939-3597-0_6.

Authors

Suni M Edson¹, Timothy P McMahon²

Affiliations

¹ Armed Forces DNA Identification Laboratory (AFDIL), American Registry of Pathology (ARP), Contractors supporting the Armed Forces Medical Examiner System (AFMES), 115 Purple Heart Drive, Dover AFB, DE, 19902, USA. suni.m.edson.ctr@mail.mil.
² Armed Forces DNA Identification Laboratory (AFDIL), American Registry of Pathology (ARP), Contractors supporting the Armed Forces Medical Examiner System (AFMES), 115 Purple Heart Drive, Dover AFB, DE, 19902, USA.

PMID: 27259732
DOI: 10.1007/978-1-4939-3597-0_6

Abstract

Acquisition of DNA from skeletal remains can be a delicate process. With the advent of improved extraction buffers that provide complete demineralization of the osseous materials, extraction of total genomic DNA from nearly any skeletal element is possible. This chapter describes both traditional organic and more newly developed inorganic extraction methods for fresh and dried skeletal remains.

Keywords: DNA from skeletal remains; Inorganic extraction; Organic extraction; PCIA.

MeSH terms

Bone and Bones / chemistry*
DNA / genetics
DNA / isolation & purification*
DNA Fingerprinting / methods*
Forensic Anthropology / methods*
Forensic Genetics / methods*
Humans

Substances

DNA