New cytotoxic and anti-inflammatory steroids from the soft coral Klyxum flaccidum

Wan-Ru Tseng; Chiung-Yao Huang; Yi-Ying Tsai; Yun-Sheng Lin; Tsong-Long Hwang; Jui-Hsin Su; Ping-Jyun Sung; Chang-Feng Dai; Jyh-Horng Sheu

doi:10.1016/j.bmcl.2016.05.060

New cytotoxic and anti-inflammatory steroids from the soft coral Klyxum flaccidum

Bioorg Med Chem Lett. 2016 Jul 15;26(14):3253-3257. doi: 10.1016/j.bmcl.2016.05.060. Epub 2016 May 24.

Authors

Wan-Ru Tseng¹, Chiung-Yao Huang¹, Yi-Ying Tsai¹, Yun-Sheng Lin², Tsong-Long Hwang³, Jui-Hsin Su⁴, Ping-Jyun Sung⁴, Chang-Feng Dai⁵, Jyh-Horng Sheu⁶

Affiliations

¹ Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
² Department of Biological Science & Technology, Mei Ho University, Pingtung 912, Taiwan.
³ Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan; Research Center for Industry of Human Ecology and Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan; Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan.
⁴ National Museum of Marine Biology & Aquarium, Pingtung 944, Taiwan.
⁵ Institute of Oceanography, National Taiwan University, Taipei 112, Taiwan.
⁶ Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan; Graduate Institute of Natural Products, School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: sheu@mail.nsysu.edu.tw.

PMID: 27256910
DOI: 10.1016/j.bmcl.2016.05.060

Abstract

Four new steroids, namely klyflaccisteroids G-J (1-4) were isolated from the Formosan soft coral Klyxum flaccidum. The structures of compounds 1-4 were established by spectral data analysis (IR, MS, 1D and 2D NMR) and comparison of spectral data with those of the related known compounds. Cytotoxicity assay revealed that 4 exhibited inhibition activity against the growth of HT-29, P388 and K562 cancer cell lines, whereas 2 showed selective cytotoxicity toward P388 cells. Compound 4 was also found to display significant anti-inflammatory activity for suppressing superoxide anion generation (O2(-)) and elastase release.

Keywords: Anti-inflammatory activity; Cytotoxicity; Klyxum flaccidum; Soft coral; Steroid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anthozoa / chemistry*
Anti-Inflammatory Agents, Non-Steroidal / chemistry
Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Antineoplastic Agents / chemistry
Antineoplastic Agents / isolation & purification
Antineoplastic Agents / pharmacology*
Cell Line, Tumor
Cell Proliferation / drug effects
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Humans
Mice
Molecular Structure
Pancreatic Elastase / antagonists & inhibitors*
Pancreatic Elastase / metabolism
Steroids / chemistry
Steroids / isolation & purification
Steroids / pharmacology*
Structure-Activity Relationship
Superoxides / antagonists & inhibitors*
Superoxides / metabolism

Substances

Anti-Inflammatory Agents, Non-Steroidal
Antineoplastic Agents
Steroids
Superoxides
Pancreatic Elastase