As an emerging field, nanotoxicology is gaining significant interest from scientists as well as from international regulatory firms in an attempt to build accumulated knowledge on this topic, which will be the basis for regulatory codes and safer nanotechnology. However, conflicting results and findings are abundant in the literature calling for more careful experimental design, result interpretation, and detailed reporting. In this perspective, we focus on misinterpretation in nanotoxicology and highlight the importance of proper experimental practice to avoid artifacts by discussing various examples from the literature.