Selection of Natural and Base-Modified DNA Aptamers for a Camptothecin Derivative

Curr Protoc Nucleic Acid Chem. 2016 Jun 1:65:9.10.1-9.10.19. doi: 10.1002/cpnc.5.

Abstract

Nucleic acid aptamers for small molecules are currently being developed and have a potential role in diverse applications including biosensing, diagnostics, and therapeutics involving low-molecular-weight biomarkers and drugs. To enhance and broaden their functions through chemical modification, systematic evolution of ligands by exponential enrichment (SELEX) selection has been attempted with modified DNA/RNA libraries. Recently, we demonstrated the superior efficacy of base modification for affinity enhancement and the usefulness of unnatural nucleic acid libraries for development of small-molecule aptamers. In this unit, we describe construction of a modified DNA library that includes (E)-5-(2-(N-(2-(N(6) -adeninyl)ethyl))carbamylvinyl)uracil bases and acquisition of high-affinity camptothecin-binding DNA aptamers, in addition to those of the corresponding natural DNA library and aptamers, using the SELEX method. © 2016 by John Wiley & Sons, Inc.

Keywords: modified DNA; nucleic acid aptamers; selex; small molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Camptothecin / chemistry*
  • Evolution, Molecular
  • Gene Library
  • Ligands
  • Small Molecule Libraries / chemistry*
  • Uracil / analogs & derivatives
  • Uracil / chemistry

Substances

  • (E)-5-(2-(N-(2-(N6-adeninyl)ethyl))carbamylvinyl)-uracil
  • Aptamers, Nucleotide
  • Ligands
  • Small Molecule Libraries
  • Uracil
  • Camptothecin