Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential

Hironaga Akita; Zen-Ichiro Kimura; Mohd Zulkhairi Mohd Yusoff; Nobutaka Nakashima; Tamotsu Hoshino

doi:10.1186/s40064-016-2237-y

Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential

Springerplus. 2016 May 11:5:596. doi: 10.1186/s40064-016-2237-y. eCollection 2016.

Authors

Hironaga Akita¹, Zen-Ichiro Kimura², Mohd Zulkhairi Mohd Yusoff³, Nobutaka Nakashima⁴, Tamotsu Hoshino⁵

Affiliations

¹ Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Sciences and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046 Japan.
² Department of Civil and Environmental Engineering, National Institute of Technology, Kure College, 2-2-11 Aga-minami, Kure, Hiroshima 737-8506 Japan.
³ Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Sciences and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046 Japan ; Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia.
⁴ Bioproduction Research Institute, National Institute of Advanced Industrial Sciences and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo, 062-8517 Japan ; Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 2-12-1-M6-5 Ookayama, Meguro-ku, Tokyo, 152-8550 Japan.
⁵ Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Sciences and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046 Japan ; Bioproduction Research Institute, National Institute of Advanced Industrial Sciences and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo, 062-8517 Japan.

Abstract

Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequencing. This strain was named CCA53, and its lignin-degrading capability was assessed by observing its growth on medium containing alkali lignin or lignin-associated aromatic monomers as the sole carbon source. Alkali lignin and at least eight lignin-associated aromatic monomers supported growth of this strain, and the most effective utilization was observed for p-hydroxybenzene monomers. These findings indicate that Burkholderia sp. strain CCA53 has fragmentary activity for lignin degradation.

Keywords: 16S rRNA gene sequencing; Burkholderia; Lignin-associated aromatic monomer; Lignin-degrading bacterium.