Developmental validation of forensic DNA-STR kits: Expressmarker 16+10Y and expressmarker 16+18Y

Huaigu Zhou; Gang Bi; Chen Zhang; Yanan Liu; Ronghua Chen; Fayuan Li; Xinglin Mei; Yulin Guo; Weiguo Zheng

doi:10.1016/j.fsigen.2016.05.011

Developmental validation of forensic DNA-STR kits: Expressmarker 16+10Y and expressmarker 16+18Y

Forensic Sci Int Genet. 2016 Sep:24:1-17. doi: 10.1016/j.fsigen.2016.05.011. Epub 2016 May 17.

Authors

Huaigu Zhou¹, Gang Bi², Chen Zhang², Yanan Liu², Ronghua Chen², Fayuan Li³, Xinglin Mei³, Yulin Guo³, Weiguo Zheng³

Affiliations

¹ Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China. Electronic address: hgzhou803@hotmail.com.
² Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China.
³ AGCU ScienTech Incorporation, Wuxi 214174, China.

PMID: 27243781
DOI: 10.1016/j.fsigen.2016.05.011

Abstract

DNA-STR analysis is widely used in the forensic science field and obtaining results in shorter time is highly demanded. The developed forensic STR Kit, referred to as Expressmarker 16+10Y (EX16+10Y) and Expressmarker 16+18Y (EX16+18Y), could amplify the common autosomal and Y chromosome STR loci simultaneously. The kits are validated by a series of tests, including DNA mixtures, stutter ratios, PCR based studies, species specificities, inhibitors, sensitivity, sizing precision, reproducibility and parallel tests. The results demonstrated that EX16+10Y and EX16+18Y were useful tools for rapid criminal investigation.

Keywords: EX16+10Y; EX16+18Y; Forensic STR kit.

Publication types

Validation Study

MeSH terms

Animals
Chromosomes, Human, Y
DNA Fingerprinting / instrumentation*
Humans
Microsatellite Repeats*
Polymerase Chain Reaction
Reproducibility of Results
Species Specificity