Spatio-temporal expression of Sox genes in murine palatogenesis

Momoko Watanabe; Katsushige Kawasaki; Maiko Kawasaki; Thantrira Portaveetus; Shelly Oommen; James Blackburn; Takahiro Nagai; Atsushi Kitamura; Atsushi Nishikawa; Yasumitsu Kodama; Ritsuo Takagi; Takeyasu Maeda; Paul T Sharpe; Atsushi Ohazama

doi:10.1016/j.gep.2016.05.002

Spatio-temporal expression of Sox genes in murine palatogenesis

Gene Expr Patterns. 2016 Jul;21(2):111-8. doi: 10.1016/j.gep.2016.05.002. Epub 2016 May 27.

Authors

Affiliations

¹ Division of Oral Anatomy, Department of Oral Biological Science, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan; Division of Oral and Maxillofacial Surgery, Department of Health Science, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan.
² Division of Oral Anatomy, Department of Oral Biological Science, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan; Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, SE1 9RT, UK; Oral Life Science, Research Center for Advanced Oral Science, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan.
³ Division of Oral Anatomy, Department of Oral Biological Science, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan; Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, SE1 9RT, UK.
⁴ Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, SE1 9RT, UK.
⁵ Division of Oral and Maxillofacial Surgery, Department of Health Science, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan.
⁶ Division of Oral Anatomy, Department of Oral Biological Science, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan.
⁷ Division of Oral Anatomy, Department of Oral Biological Science, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan; Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, SE1 9RT, UK. Electronic address: atsushiohazama@dent.niigata-u.ac.jp.

PMID: 27241892
DOI: 10.1016/j.gep.2016.05.002

Abstract

Members of the Sox gene family play critical roles in many biological processes including organogenesis. We carried out comparative in situ hybridisation analysis of seventeen Sox genes (Sox1-14, 17, 18 and 21) during murine palatogenesis from initiation to fusion of the palatal shelves above the dorsal side of the tongue. At palatal shelf initiation (E12.5), the localized expression of six Sox genes (Sox2, 5, 6, 9, 12 and 13) was observed in the shelves, whereas Sox4 and Sox11 showed ubiquitious expression. During the down-growth of palatal shelves (E13.5), Sox4, Sox5, and Sox9 exhibited restricted expression to the interior side of the palatal shelves facing the tongue. Following elevation of the palatal shelves (E14.5), Sox2, Sox11 and Sox21 expression was present in the midline epithelial seam. We thus identify dynamic spatio-temporal expression of Sox gene family during the process of palatogenesis.

Keywords: In situ hybridisation; Palatal development; Sox.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Epithelial Cells / metabolism
Gene Expression Regulation, Developmental
In Situ Hybridization
Mice
Multigene Family / genetics
Organogenesis / genetics*
Palate / growth & development
Palate / metabolism*
SOX Transcription Factors / biosynthesis
SOXB1 Transcription Factors / biosynthesis*
SOXB2 Transcription Factors / biosynthesis*
SOXC Transcription Factors / biosynthesis*

Substances

SOX Transcription Factors
SOXB1 Transcription Factors
SOXB2 Transcription Factors
SOXC Transcription Factors
Sox11 protein, mouse
Sox2 protein, mouse
Sox21 protein, mouse