Objective: To observe the effect of Van-Clear on vamplification of human telomerase RNA component (hTERC) gene in cervical tissues by fluorescence in situ hybridization, and to determine the potential for Van-Clear to replace xylene.
Methods: A total of 278 specimens of cervix uteri were collected from inpatients of Department of Gynaecology in Boai Hospital of Zhongshan from January to February, 2015, with 81 cases of normal specimens, 68 cases of cervical intraepithelial neoplasia (CIN) I, 57cases of CIN2, 42 cases of CIN3 and 30 cases of cervical invasive cancer. Double samples were collected from the same region. Fluorescence in situ hybridization was applied to detect the changes in the amplification of hTERC gene in 2 groups of specimens from the cervical biopsy.
Results: Differences in the positive expression rate of hTERC gene between the 2 groups of cervical lesions at all levels were not statistically significant (P>0.05).
Conclusion: There is no significant difference in the positive rate of hTERC gene expression between the slices made by Van-clear and xylene. As an environmental-friend product, Van-Clear possesses certain value in detection of cervical hTERC gene by fluorescence in situ hybridization.
目的：观察Van-clear(环保透明脱蜡液)替代二甲苯(传统透明脱蜡液)在荧光原位杂交技术检测宫颈组织中人端粒酶核糖核酸组分(human telomerase RNA component，hTERC)基因扩增中的结果，探讨其替代二甲苯的可行性。方法：收集2005年1月至2月于中山市博爱医院妇科住院部送检的278例宫颈组织标本。包括正常组81例，宫颈上皮内瘤变(cervical intraepithelial neoplasia，CIN)1级组68例，CIN2级组57例，CIN3级／原位癌组42例，宫颈浸润癌组30例。同一病变部位取材2块，分两组，一组采用二甲苯透明制作切片278张；另一组采用Van-clear透明制作切片278张。采用荧光原位杂交技术检测两组宫颈活检标本中hTERC基因的扩增变化情况。结果：两组间对应的各级宫颈病变中，hTERC基因阳性表达率差异均无统计学意义(均P>0.05)。结论：用Van-Clear替代二甲苯对荧光原位杂交技术检测宫颈组织hTERC基因阳性表达率无明显影响，Van-clear作为环保产品,在荧光原位杂交技术检测宫颈hTERC基因中有一定的推广应用价值。.