Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems

Yin Hui Chow; Yee Jiun Yap; Pau Loke Show; Joon Ching Juan; Mohd Shamsul Anuar; Eng-Poh Ng; Chien-Wei Ooi; Tau Chuan Ling

doi:10.1016/j.jbiosc.2016.04.008

Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems

J Biosci Bioeng. 2016 Nov;122(5):613-619. doi: 10.1016/j.jbiosc.2016.04.008. Epub 2016 May 24.

Authors

Yin Hui Chow¹, Yee Jiun Yap², Pau Loke Show³, Joon Ching Juan⁴, Mohd Shamsul Anuar⁵, Eng-Poh Ng⁶, Chien-Wei Ooi⁷, Tau Chuan Ling⁸

Affiliations

¹ Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; School of Engineering, Taylor's University, No. 1 Jalan Taylor's, 47500 Subang Jaya, Selangor, Malaysia.
² Department of Applied Mathematics, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
³ Food and Pharmaceutical Engineering Research Group, Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
⁴ Nanotechnology & Catalyst Research Centre (NANOCAT), University of Malaya, 50603 Kuala Lumpur, Malaysia.
⁵ Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
⁶ School of Chemical Sciences, Universiti Sains Malaysia, Minden 11800, Malaysia.
⁷ Chemical Engineering Discipline and Advanced Engineering Platform, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia. Electronic address: ooi.chien.wei@monash.edu.
⁸ Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

PMID: 27233672
DOI: 10.1016/j.jbiosc.2016.04.008

Abstract

The partitioning behavior of immunoglobulin G (IgG) in the aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and phosphate was studied. The parameters of ATPS exhibiting the pronounced effects on the partitioning behavior of IgG include phase composition, PEG molecular weight, and the addition of sodium chloride (NaCl). The accumulation of IgG at the interface of the ATPS increased drastically as the tie-line length (TLL) was increased. This trend was correlated with a linear relationship relating the natural logarithm of interfacial partition coefficient (ln G) to the difference of PEG concentration between the top phase and the bottom phase (Δ[PEG]), and a good fit was obtained. An attempt was made to correlate the natural logarithm of partition coefficient (ln K) to the presence of NaCl with the proposed linear relationship, ln K = α″ ln [Cl^-] + β″. The proposed relationship, which serves as a better description of the underlying mechanics of the protein partitioning behavior in the polymer-salt ATPS, provides a good fit (r² > 0.95) for the data of IgG partitioning. An optimum recovery of 99.97% was achieved in an ATPS (pH 7.5) composed of 14.0% (w/w) PEG 1450, 12.5% (w/w) phosphate and 5.0% (w/w) NaCl.

Keywords: Aqueous two-phase system; Bioseparation; Immunoglobulin G; Protein recovery; Purification; Statistical mechanics.

MeSH terms

Chemical Fractionation / methods*
Humans
Immunoglobulin G / chemistry*
Molecular Weight
Phosphates
Polyethylene Glycols / chemistry
Polymers / chemistry*
Sodium Chloride / chemistry*
Water / chemistry*

Substances

Immunoglobulin G
Phosphates
Polymers
Water
Polyethylene Glycols
Sodium Chloride