The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous substrate. A suitable incubation period results in the formation of plaques, which can be fixed and stained for visualization. Here, we describe a method for measuring Chikungunya virus (CHIKV) titers via virus plaque assays.
Keywords: Aquacide; Carboxymethyl cellulose; Chikungunya virus; Crystal violet; Plaque assays; Virus titration.