Optical fiber LPG biosensor integrated microfluidic chip for ultrasensitive glucose detection

Ming-Jie Yin; Bobo Huang; Shaorui Gao; A Ping Zhang; Xuesong Ye

doi:10.1364/BOE.7.002067

Optical fiber LPG biosensor integrated microfluidic chip for ultrasensitive glucose detection

Biomed Opt Express. 2016 Apr 28;7(5):2067-77. doi: 10.1364/BOE.7.002067. eCollection 2016 May 1.

Authors

Ming-Jie Yin¹, Bobo Huang², Shaorui Gao¹, A Ping Zhang¹, Xuesong Ye³

Affiliations

¹ Photonics Research Center, Department of Electrical Engineering, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, China.
² Photonics Research Center, Department of Electrical Engineering, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, China; Biosensor National Special Laboratory, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China.
³ Biosensor National Special Laboratory, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China.

Abstract

An optical fiber sensor integrated microfluidic chip is presented for ultrasensitive detection of glucose. A long-period grating (LPG) inscribed in a small-diameter single-mode fiber (SDSMF) is employed as an optical refractive-index (RI) sensor. With the layer-by-layer (LbL) self-assembly technique, poly (ethylenimine) (PEI) and poly (acrylic acid) (PAA) multilayer film is deposited on the SDSMF-LPG sensor for both supporting and signal enhancement, and then a glucose oxidase (GOD) layer is immobilized on the outer layer for glucose sensing. A microfluidic chip for glucose detection is fabricated after embedding the SDSMF-LPG biosensor into the microchannel of the chip. Experimental results reveal that the SDSMF-LPG biosensor based on such a hybrid sensing film can ultrasensitively detect glucose concentration as low as 1 nM. After integration into the microfluidic chip, the detection range of the sensor is extended from 2 µM to 10 µM, and the response time is remarkablely shortened from 6 minutes to 70 seconds.

Keywords: (050.2770) Gratings; (060.2370) Fiber optics sensors; (160.1435) Biomaterials; (280.1415) Biological sensing and sensors.