PEG stabilized DNA - poly(ferrocenylsilane) polyplexes for gene delivery

B Zoetebier; A Sohrabi; B Lou; M A Hempenius; W E Hennink; G J Vancso

doi:10.1039/c6cc02733d

PEG stabilized DNA - poly(ferrocenylsilane) polyplexes for gene delivery

Chem Commun (Camb). 2016 Jun 8;52(49):7707-10. doi: 10.1039/c6cc02733d.

Authors

B Zoetebier¹, A Sohrabi², B Lou³, M A Hempenius¹, W E Hennink³, G J Vancso¹

Affiliations

¹ Department of Materials Science and Technology of Polymers, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. G.J.Vancso@utwente.nl.
² Department of Materials Science and Technology of Polymers, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. G.J.Vancso@utwente.nl and Department of Bioengineering, University of California, 420 Westwood Plaza, 5121 Engineering V, P.O. Box 951600, Los Angeles, CA 90095, USA.
³ Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CG Utrecht, The Netherlands. W.E.Hennink@uu.nl.

PMID: 27230678
DOI: 10.1039/c6cc02733d

Abstract

Polycationic poly(ferrocenylsilane)s (PFS) with tunable amounts of PEG side chains were used for the condensation of DNA into polyplexes of 110 nm in 5.0 mM HEPES. The PFS-PEG/DNA polyplexes showed negligible aggregation, a strongly reduced protein adsorption, transfection activities comparable with linear polyethyleneimine and an excellent cytocompatibility.

MeSH terms

DNA / chemistry*
DNA / genetics*
Drug Carriers / chemistry*
Drug Carriers / pharmacology
Ferrous Compounds / chemistry*
Materials Testing
Polyethyleneimine / chemistry*
Silanes / chemistry*
Transfection*

Substances

Drug Carriers
Ferrous Compounds
Silanes
poly(ferrocenylsilane)
Polyethyleneimine
DNA