G-quadruplex vs. duplex-DNA binding of nickel(II) and zinc(II) Schiff base complexes

Riccardo Bonsignore; Alessio Terenzi; Angelo Spinello; Annamaria Martorana; Antonino Lauria; Anna Maria Almerico; Bernhard K Keppler; Giampaolo Barone

doi:10.1016/j.jinorgbio.2016.05.010

G-quadruplex vs. duplex-DNA binding of nickel(II) and zinc(II) Schiff base complexes

J Inorg Biochem. 2016 Aug:161:115-21. doi: 10.1016/j.jinorgbio.2016.05.010. Epub 2016 May 14.

Authors

Riccardo Bonsignore¹, Alessio Terenzi², Angelo Spinello¹, Annamaria Martorana³, Antonino Lauria³, Anna Maria Almerico³, Bernhard K Keppler², Giampaolo Barone⁴

Affiliations

¹ Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Viale delle Scienze, Edificio 17, 90128, Palermo, Italy; Istituto EuroMediterraneo di Scienza e Tecnologia, Piazzetta Briuccia, 20-21, 90139, Palermo, Italy.
² Institute of Inorganic Chemistry, University of Vienna, Waehringerstr. 42, A-1090 Vienna, Austria; Research Platform "Translational Cancer Therapy Research", University of Vienna and Medical University of Vienna, Vienna, Austria.
³ Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Viale delle Scienze, Edificio 17, 90128, Palermo, Italy.
⁴ Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Viale delle Scienze, Edificio 17, 90128, Palermo, Italy; Istituto EuroMediterraneo di Scienza e Tecnologia, Piazzetta Briuccia, 20-21, 90139, Palermo, Italy. Electronic address: giampaolo.barone@unipa.it.

PMID: 27230387
DOI: 10.1016/j.jinorgbio.2016.05.010

Abstract

Novel nickel(II) (1) and zinc(II) (2) complexes of a Salen-like ligand, carrying a pyrimidine ring on the N,N' bridge, were synthesized and characterized. Their interaction with duplex and G-quadruplex DNA was investigated in aqueous solution through UV-visible absorption, circular dichroism and viscometry measurements. The results obtained point out that, while the zinc(II) complex does not interact with both duplex and G-quadruplex DNA, the nickel(II) complex 1 binds preferentially to G-quadruplex respect to duplex-DNA, with values of the DNA-binding constants, Kb, 2.6×10(5)M(-1) and 3.5×10(4)M(-1), respectively. Molecular dynamics simulations provided an atomic level model of the top-stacking binding occurring between 1 and hTelo (a 22-mer sequence oligonucleotide) in G-quadruplex conformation.

Keywords: Binding constant; Circular dichroism; Computational chemistry; DNA G-quadruplex, nickel; zinc.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
G-Quadruplexes*
Models, Molecular*
Nickel / chemistry*
Schiff Bases / chemistry
Zinc / chemistry*

Substances

Schiff Bases
Nickel
DNA
Zinc