Tegument Protein ORF45 Plays an Essential Role in Virion Morphogenesis of Murine Gammaherpesvirus 68

Xing Jia; Sheng Shen; Ying Lv; Ziwei Zhang; Haitao Guo; Hongyu Deng

doi:10.1128/JVI.03231-15

Tegument Protein ORF45 Plays an Essential Role in Virion Morphogenesis of Murine Gammaherpesvirus 68

J Virol. 2016 Jul 27;90(16):7587-7592. doi: 10.1128/JVI.03231-15. Print 2016 Aug 15.

Authors

Xing Jia^{1

2}, Sheng Shen¹, Ying Lv^{1

2}, Ziwei Zhang^{1

2}, Haitao Guo¹, Hongyu Deng^{3

4}

Affiliations

¹ Chinese Academy of Sciences Key Laboratory of Infection and Immunity, Institute of Biophysics, Beijing, People's Republic of China.
² University of Chinese Academy of Sciences, Beijing, People's Republic of China.
³ Chinese Academy of Sciences Key Laboratory of Infection and Immunity, Institute of Biophysics, Beijing, People's Republic of China hydeng@moon.ibp.ac.cn.
⁴ CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, People's Republic of China.

Abstract

Tegument proteins play critical roles in herpesvirus morphogenesis. ORF45 is a conserved tegument protein of gammaherpesviruses; however, its role in virion morphogenesis is largely unknown. In this work, we determined the ultrastructural localization of murine gammaherpesvirus 68 (MHV-68) ORF45 and found that this protein was incorporated into virions around the site of host-derived vesicles. Notably, the absence of ORF45 inhibited nucleocapsid egress and blocked cytoplasmic virion maturation, demonstrating that ORF45 is essential for MHV-68 virion morphogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Cricetinae
Epithelial Cells
Immediate-Early Proteins / metabolism*
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Morphogenesis*
Rhadinovirus / physiology*
Virion / metabolism*
Virus Assembly
Virus Release

Substances

Immediate-Early Proteins
ORF45 protein, murine gammaherpesvirus 68