In vitro selection of antibiotic-binding aptamers

Florian Groher; Beatrix Suess

doi:10.1016/j.ymeth.2016.05.008

In vitro selection of antibiotic-binding aptamers

Methods. 2016 Aug 15:106:42-50. doi: 10.1016/j.ymeth.2016.05.008. Epub 2016 May 17.

Authors

Florian Groher¹, Beatrix Suess²

Affiliations

¹ Department of Biology, Technical University Darmstadt, Schnittspahnstr. 10, 64287 Darmstadt, Germany.
² Department of Biology, Technical University Darmstadt, Schnittspahnstr. 10, 64287 Darmstadt, Germany. Electronic address: bsuess@bio.tu-darmstadt.de.

PMID: 27223401
DOI: 10.1016/j.ymeth.2016.05.008

Abstract

Despite its wide applicability the selection of small molecule-binding RNA aptamers with high affinity binding and specificity is still challenging. We will present here a protocol which allows the in vitro selection of antibiotic-binding aptamers which turned out to be important building blocks for the design process of synthetic riboswitches. The presented methods will be compared with alternative in vitro selection protocols. A detailed note section will point out useful tips and pitfalls.

Keywords: Biotechnology; Combinatorial chemistry; In vitro selection; RNA aptamer; SELEX.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemistry*
Aptamers, Nucleotide / chemistry
Aptamers, Nucleotide / genetics*
Riboswitch / genetics
SELEX Aptamer Technique / methods*

Substances

Anti-Bacterial Agents
Aptamers, Nucleotide
Riboswitch