Haemolysates from 1098 unrelated individuals residing at Lucknow were investigated for haemoglobin variants by cellogel electrophoresis. Haemoglobin 'D' trait was found in 16 subjects (1.5%). The prevalence of Hb-D trait in Khatris was 3.1 per cent compared to 0.5 per cent in other Hindus. One case of Hb-D trait with thaiassaemia was also seen in a Muslim girl.