Assessing Caspase-1 Activation

Methods Mol Biol. 2016:1417:197-206. doi: 10.1007/978-1-4939-3566-6_13.

Abstract

The caspase-1 enzymatic activity plays a major role in the innate immune response as it regulates the maturation of two major proinflammatory cytokines, the interleukin-1beta (IL-1β) and IL-18. In this chapter, we describe the technique of Western blot to assess caspase-1 activation. This method provides multiple information within one experiment. It allows the detection of both unprocessed and processed caspase-1 and substrates.

Keywords: Caspase-1; Inflammasome; Interleukin-1beta (IL-1β); Western blot.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Caspase 1 / metabolism*
  • Cells, Cultured
  • Immunity, Innate
  • Interleukin-18 / metabolism*
  • Interleukin-1beta / metabolism*
  • Macrophages, Peritoneal / cytology
  • Macrophages, Peritoneal / metabolism
  • Mice

Substances

  • IL1B protein, mouse
  • Interleukin-18
  • Interleukin-1beta
  • Caspase 1