The latitudinal diversity gradient (LDG) has been one of the most documented patterns in ecology, typically showing decreasing species diversity with increasing latitude. Studies of these patterns also used different spatial scales and dispersal traits to better understand the underpinning ecological factors. Seasonal freshwater ecosystems are less studied and may exhibit different patterns because they are more sensitive to climatic variation, which result in an inundation-desiccation cycle. In California, precipitation increases and temperature decreases with increasing latitude and thus the LDG pattern may be associated with this climatic gradient. Using collected data and United States Fish and Wildlife Service reports across seven degrees of latitude, analysis of California vernal pool invertebrate community (total richness and richness of passive and active dispersers) was conducted using correlations (Spearman rank and partial). Alpha diversity (total and passive dispersers) increased and beta diversity (passive dispersers) decreased with increasing latitude. Vernal pool surface area was correlated with active disperser alpha and passive disperser beta diversity. This suggests that climate-driven habitat size influences alpha and beta diversity patterns depending on dispersal ability. Active dispersers and predators exhibited higher beta diversity than passive dispersers and prey, respectively. Species composition differed among counties and some of these differences were correlated with pool depth and temperature. These results suggest that seasonal habitats will have diversity patterns strongly associated with local scale characteristics (habitat size and hydroperiod) determined by climate variation along the latitudinal gradient. Understanding these diversity patterns along the gradient will also contribute to management and restoration of these ecosystems with high endemism and diversity.