Hybrid Mass Spectrometry: Towards Characterization of Protein Conformational States

Zainab Ahdash; Euan Pyle; Argyris Politis

doi:10.1016/j.tibs.2016.04.008

Hybrid Mass Spectrometry: Towards Characterization of Protein Conformational States

Trends Biochem Sci. 2016 Aug;41(8):650-653. doi: 10.1016/j.tibs.2016.04.008.

Authors

Zainab Ahdash¹, Euan Pyle², Argyris Politis³

Affiliations

¹ Department of Chemistry, King's College London, London, UK.
² Department of Chemistry, King's College London, London, UK; Department of Chemistry, Imperial College London, London, UK.
³ Department of Chemistry, King's College London, London, UK. Electronic address: argyris.politis@kcl.ac.uk.

PMID: 27211036
DOI: 10.1016/j.tibs.2016.04.008

Abstract

A current challenge in structural biology is to unravel the conformational states of protein complexes. Hybrid mass spectrometry (MS) has emerged as a key tool to study the structural dynamics of large protein complexes unattainable by traditional methods. Here, we discuss recent advances in hybrid MS allowing characterization of challenging biological systems.

Keywords: conformational states; hybrid approaches; integrative modelling; mass spectrometry; protein complexes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Mass Spectrometry / methods*
Protein Conformation
Proteins / chemistry*

Substances

Proteins

Grants and funding

Wellcome Trust/United Kingdom