AtSRP1, SMALL RUBBER PARTICLE PROTEIN HOMOLOG, functions in pollen growth and development in Arabidopsis

Biochem Biophys Res Commun. 2016 Jun 24;475(2):223-9. doi: 10.1016/j.bbrc.2016.05.083. Epub 2016 May 18.

Abstract

To identify novel roles of SMALL RUBBER PARTICLE PROTEIN Homolog in the non-rubber-producing plant Arabidopsis (AtSRP1), we isolated a T-DNA-insertion knock-out mutant (FLAG_543A05) and investigated its functional characteristics. AtSRP1 is predominantly expressed in reproductive organs and is localized to lipid droplets and ER. Compared to wild-type (WT) Arabidopsis, atsrp1 plants contain small siliques with a reduced number of heterogeneously shaped seeds. The size of anther and pollen grains in atsrp1 is highly irregular, with a lower grain number than WT. Therefore, AtSRP1 plays a novel role related to pollen growth and development in a non-rubber-producing plant.

Keywords: Non-rubber-producing plant; Physiological function; Pollen growth and development; SMALL RUBBER PARTICLE PROTEIN HOMOLOG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / analysis
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Endoplasmic Reticulum / genetics
  • Endoplasmic Reticulum / metabolism
  • Gene Knockout Techniques
  • Mutation
  • Pollen / genetics
  • Pollen / growth & development*
  • Pollen / metabolism
  • Seeds / genetics
  • Seeds / growth & development*
  • Seeds / metabolism

Substances

  • Arabidopsis Proteins
  • SRP1 protein, Arabidopsis