Development of a real-time PCR assay for quantification of Citrobacter rodentium

Sofia Sagaidak; Amel Taibi; Bijun Wen; Elena M Comelli

doi:10.1016/j.mimet.2016.05.008

Development of a real-time PCR assay for quantification of Citrobacter rodentium

J Microbiol Methods. 2016 Jul:126:76-7. doi: 10.1016/j.mimet.2016.05.008. Epub 2016 May 16.

Authors

Sofia Sagaidak¹, Amel Taibi¹, Bijun Wen¹, Elena M Comelli²

Affiliations

¹ Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, ON, Canada.
² Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, ON, Canada; Centre for Child Nutrition and Health, Faculty of Medicine, University of Toronto, ON, Canada. Electronic address: elena.comelli@utoronto.ca.

PMID: 27196638
DOI: 10.1016/j.mimet.2016.05.008

Abstract

Molecular tools to quantify Citrobacter rodentium are not available. We developed a quantitative PCR assay targeting the espB gene. This assay is specific, has a linearity range of about 6.7×10(1) to 6.7×10(6)cells/PCR reaction (92% efficiency) and a detection limit of about 10(4)cells/g wet feces.

Keywords: Citrobacter rodentium; Mice; espB; qPCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Load / methods*
Bacterial Outer Membrane Proteins / genetics
Citrobacter rodentium / genetics
Citrobacter rodentium / isolation & purification*
DNA, Bacterial
Feces / microbiology*
Mice
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity

Substances

Bacterial Outer Membrane Proteins
DNA, Bacterial