Structural characterization of the lipoteichoic acid isolated from Staphylococcus sciuri W620

Katarzyna A Duda; Sandra Petersen; Otto Holst

doi:10.1016/j.carres.2016.04.026

Structural characterization of the lipoteichoic acid isolated from Staphylococcus sciuri W620

Carbohydr Res. 2016 Jul 22:430:44-47. doi: 10.1016/j.carres.2016.04.026. Epub 2016 May 4.

Authors

Katarzyna A Duda¹, Sandra Petersen², Otto Holst²

Affiliations

¹ Junior Research Group Allergobiochemistry. Electronic address: kduda@fz-borstel.de.
² Division of Structural Biochemistry, Priority Area Asthma and Allergies, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Parkallee 4a/c, D-23845 Borstel, Germany.

PMID: 27196311
DOI: 10.1016/j.carres.2016.04.026

Abstract

Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria. LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments. Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids. The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.

Keywords: Allergy-protection; LTA structure; NMR analysis; Staphylococcus sciuri.

MeSH terms

Carbohydrate Sequence
Lipopolysaccharides / chemistry*
Staphylococcus / chemistry*
Teichoic Acids / chemistry*

Substances

Lipopolysaccharides
Teichoic Acids
lipoteichoic acid