Functional analysis of the N-terminal basic motif of a eukaryotic satellite RNA virus capsid protein in replication and packaging

Sci Rep. 2016 May 19:6:26328. doi: 10.1038/srep26328.

Abstract

Efficient replication and assembly of virus particles are integral to the establishment of infection. In addition to the primary role of the capsid protein (CP) in encapsidating the RNA progeny, experimental evidence on positive sense single-stranded RNA viruses suggests that the CP also regulates RNA synthesis. Here, we demonstrate that replication of Satellite tobacco mosaic virus (STMV) is controlled by the cooperative interaction between STMV CP and the helper virus (HV) Tobacco mosaic virus (TMV) replicase. We identified that the STMV CP-HV replicase interaction requires a positively charged residue at the third position (3R) in the N-terminal 13 amino acid (aa) motif. Far-Northwestern blotting showed that STMV CP promotes binding between HV-replicase and STMV RNA. An STMV CP variant having an arginine to alanine substitution at position 3 in the N-terminal 13aa motif abolished replicase-CP binding. The N-terminal 13aa motif of the CP bearing alanine substitutions for positively charged residues located at positions 5, 7, 10 and 11 are defective in packaging full-length STMV, but can package a truncated STMV RNA lacking the 3' terminal 150 nt region. These findings provide insights into the mechanism underlying the regulation of STMV replication and packaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium / virology
  • Amino Acid Motifs
  • Capsid Proteins / metabolism*
  • Helper Viruses / physiology
  • Nicotiana / virology
  • RNA, Viral / biosynthesis
  • RNA-Dependent RNA Polymerase / metabolism
  • Tobacco Mosaic Virus / enzymology
  • Tobacco mosaic satellite virus / physiology*
  • Virion
  • Virus Assembly
  • Virus Replication

Substances

  • Capsid Proteins
  • RNA, Viral
  • tobacco mosaic virus replicase
  • RNA-Dependent RNA Polymerase