Enhanced Lineage-Specific Differentiation Efficiency of Human Induced Pluripotent Stem Cells by Engineering Colony Dimensionality Using Electrospun Scaffolds

Maricela Maldonado; Gerardo Ico; Karen Low; Rebeccah J Luu; Jin Nam

doi:10.1002/adhm.201600141

Enhanced Lineage-Specific Differentiation Efficiency of Human Induced Pluripotent Stem Cells by Engineering Colony Dimensionality Using Electrospun Scaffolds

Adv Healthc Mater. 2016 Jun;5(12):1408-12. doi: 10.1002/adhm.201600141. Epub 2016 May 17.

Authors

Maricela Maldonado¹, Gerardo Ico¹, Karen Low¹, Rebeccah J Luu¹, Jin Nam¹

Affiliation

¹ Department of Bioengineering, University of California-Riverside, Materials Science & Engineering Building 331, 900 University Avenue, Riverside, CA, 92521, USA.

PMID: 27187808
DOI: 10.1002/adhm.201600141

Abstract

Electrospun scaffolds with varied stiffness promote distinct colony morphology of human induced pluripotent stem cells, which affects their subsequent differentiation. On soft scaffolds, induced pluripotent stem cells develop 3D colonies due to the pliability of the electrospun fibrous networks, leading to greater differentiation tendency to ectodermal lineage.

Keywords: differentiation; electrospun scaffolds; induced pluripotent stem cells; mechanical stem cell niche.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation*
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Tissue Scaffolds / chemistry*