A simultaneous two-colour detection method of human IgG- and IgE-reactive proteins from lactic acid bacteria

Lidia Hanna Markiewicz; Agata Szymkiewicz; Anna Szyc; Barbara Wróblewska

doi:10.1016/j.mimet.2016.05.007

A simultaneous two-colour detection method of human IgG- and IgE-reactive proteins from lactic acid bacteria

J Microbiol Methods. 2016 Jul:126:72-5. doi: 10.1016/j.mimet.2016.05.007. Epub 2016 May 13.

Authors

Lidia Hanna Markiewicz¹, Agata Szymkiewicz², Anna Szyc², Barbara Wróblewska²

Affiliations

¹ Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Str. 10, 10-748 Olsztyn, Poland. Electronic address: l.markiewicz@pan.olsztyn.pl.
² Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Str. 10, 10-748 Olsztyn, Poland.

PMID: 27184086
DOI: 10.1016/j.mimet.2016.05.007

Abstract

Whole cell extracts of two Lactobacillus strains were tested with primary antibodies from two pooled sera from allergic patients. Fluorescently labelled anti-human IgG and anti-human IgE secondary antibodies applied in Western blotting, together with an appropriate image acquisition protocol facilitated imagining bacterial proteins that reacted with human IgG and IgE.

Keywords: Bacterial proteins; Human serum; IgE; IgG; Immunoreactivity; Lactobacillus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / chemistry
Bacterial Proteins / immunology*
Bacterial Proteins / isolation & purification*
Blotting, Western / methods*
Fluorescent Dyes
Humans
Hypersensitivity
Immunoglobulin E / immunology*
Immunoglobulin G / immunology*
Lacticaseibacillus casei / chemistry
Lactobacillus / chemistry*
Lactobacillus delbrueckii / chemistry

Substances

Bacterial Proteins
Fluorescent Dyes
Immunoglobulin G
Immunoglobulin E