Absence of chromosomal translocations and protein expression of ALK in sinonasal adenocarcinomas

Esteban Pacheco; José Luis Llorente; Alejandro López-Hernández; Cristina García-Inclán; María Costales; Sira Potes Ares; Fernando López; Blanca Vivanco; Mario A Hermsen

doi:10.1016/j.otorri.2016.02.003

Absence of chromosomal translocations and protein expression of ALK in sinonasal adenocarcinomas

Acta Otorrinolaringol Esp. 2017 Jan-Feb;68(1):9-14. doi: 10.1016/j.otorri.2016.02.003. Epub 2016 May 13.

[Article in English, Spanish]

Authors

Esteban Pacheco¹, José Luis Llorente², Alejandro López-Hernández¹, Cristina García-Inclán¹, María Costales¹, Sira Potes Ares¹, Fernando López¹, Blanca Vivanco³, Mario A Hermsen¹

Affiliations

¹ Departamento de Otorrinolaringología, Instituto Universitario de Oncología del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo, Asturias, España.
² Departamento de Otorrinolaringología, Instituto Universitario de Oncología del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo, Asturias, España. Electronic address: llorentependas@telefonica.net.
³ Departamento de Anatomía Patológica, Hospital Universitario Central de Asturias, Oviedo, Asturias, España.

PMID: 27183864
DOI: 10.1016/j.otorri.2016.02.003

Abstract

Introduction: Chromosomal translocations at 2p23 cause overexpression of anaplastic lymphoma kinase (ALK), a receptor tyrosine kinase involved in signalling pathways that regulate cell proliferation. This translocation occurs in 5% of lung adenocarcinoma and has been demonstrated to be useful as a therapeutic target for crizotinib. sinonasal adenocarcinomas (SNAC) are histologically similar to lung adenocarcinomas; the aim of this study was to evaluate the presence of ALK alterations in SNAC.

Method: Break-apart fluorescent in-situ hybridization was used to analyse the presence of ALK translocations in 96 tumour samples. In addition, ALK protein expression was studied by immunohistochemistry.

Results: The samples of SNAC did not show ALK translocation. Moreover, ALK protein expression was absent in all cases.

Conclusions: These results suggest that ALK is not involved in SNAC.

Keywords: ALK; Adenocarcinoma nasosinusal; Carcinoma nasosinusal; FISH; Sinonasal adenocarcinoma; Sinonasal carcinoma.

MeSH terms

Adenocarcinoma / genetics*
Adenocarcinoma / metabolism
Adenocarcinoma / pathology
Aged
Aged, 80 and over
Anaplastic Lymphoma Kinase
Chromosomes, Human, Pair 2 / genetics
Chromosomes, Human, Pair 2 / ultrastructure*
Crizotinib
Female
Follow-Up Studies
Gene Expression Regulation, Neoplastic*
Humans
In Situ Hybridization, Fluorescence
Male
Middle Aged
Neoplasm Proteins / biosynthesis
Neoplasm Proteins / genetics*
Nose Neoplasms / chemistry
Nose Neoplasms / genetics*
Nose Neoplasms / metabolism
Nose Neoplasms / pathology
Paranasal Sinus Neoplasms / genetics*
Paranasal Sinus Neoplasms / metabolism
Paranasal Sinus Neoplasms / pathology
Pyrazoles
Pyridines
Receptor Protein-Tyrosine Kinases / biosynthesis
Receptor Protein-Tyrosine Kinases / genetics*
Translocation, Genetic*

Substances

Neoplasm Proteins
Pyrazoles
Pyridines
Crizotinib
ALK protein, human
Anaplastic Lymphoma Kinase
Receptor Protein-Tyrosine Kinases