Photoinduced apoptosis using a peptide carrying a photosensitizer

Bioorg Med Chem Lett. 2016 Jul 1;26(13):3115-3118. doi: 10.1016/j.bmcl.2016.04.091. Epub 2016 Apr 30.

Abstract

A novel molecule, TatBim-Alexa, consisting of the HIV1 Tat cell-penetrating peptide, the Bim apoptosis-inducing peptide, and Alexa Fluor 546 was synthesized for photoinducion of apoptosis. The Alexa Fluor 546 was used as a photosensitizer and covalently attached at the C-terminus of TatBim peptide by the thiol-maleimide reaction. Photo-dependent cytosolic internalization of TatBim-Alexa and photo-dependent apoptosis using TatBim-Alexa were demonstrated in several kinds of mammalian cells including human cancer cell lines.

Keywords: Apoptosis; Bim; Cell-penetrating peptide; Photosensitizer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects*
  • CHO Cells
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cricetulus
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / pharmacology*
  • HeLa Cells
  • Humans
  • Molecular Structure
  • Peptides / chemistry
  • Peptides / pharmacology*
  • Photochemical Processes
  • Photosensitizing Agents / chemical synthesis
  • Photosensitizing Agents / chemistry
  • Photosensitizing Agents / pharmacology*
  • Quinolinium Compounds / chemical synthesis
  • Quinolinium Compounds / chemistry
  • Quinolinium Compounds / pharmacology*
  • Structure-Activity Relationship

Substances

  • Alexa fluor 546
  • Antineoplastic Agents
  • Fluorescent Dyes
  • Peptides
  • Photosensitizing Agents
  • Quinolinium Compounds