Role of L1CAM in the Regulation of the Canonical Wnt Pathway and Class I MAGE Genes

M Yu Shkurnikov; E N Knyazev; D Wicklein; U Schumacher; T R Samatov; A G Tonevitskii

doi:10.1007/s10517-016-3315-4

Role of L1CAM in the Regulation of the Canonical Wnt Pathway and Class I MAGE Genes

Bull Exp Biol Med. 2016 Apr;160(6):807-10. doi: 10.1007/s10517-016-3315-4. Epub 2016 May 10.

Authors

M Yu Shkurnikov¹, E N Knyazev², D Wicklein³, U Schumacher³, T R Samatov⁴, A G Tonevitskii²

Affiliations

¹ National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia. mshkurnikov@imscs.msu.ru.
² National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.
³ University Medical-Center Hamburg-Eppendorf, Hamburg, Germany.
⁴ Moscow State University of Mechanical Engineering (MAMI), Moscow, Russia.

PMID: 27165065
DOI: 10.1007/s10517-016-3315-4

Abstract

Molecule L1CAM is specific for nerve cells and tumors of various localizations. The expression of L1CAM is significantly higher in melanoma in comparison with benign nevi and correlates with the progress of melanoma and transition from radial to vertical growth. Monoclonal antibodies to L1CAM effectively and specifically attenuate melanoma growth, though stimulates the epithelial-mesenchymal transition. shRNA-mediated knock-down of L1CAM showed the involvement of L1CAM in regulation of activity of the canonical Wnt pathway and expression of genes of class I melanoma-associated antigens (MAGE).

Keywords: L1CAM; MAGE; Wnt; melanoma; shRNA.

MeSH terms

Antigens, Neoplasm / genetics*
Antigens, Neoplasm / metabolism
Cell Line, Tumor
Gene Expression
Gene Expression Regulation, Neoplastic
Humans
Melanoma / genetics
Melanoma / metabolism
Neoplasm Proteins / genetics*
Neoplasm Proteins / metabolism
Neural Cell Adhesion Molecule L1 / physiology*
Wnt Signaling Pathway*

Substances

Antigens, Neoplasm
MAGEC1 protein, human
MAGEC2 protein, human
Neoplasm Proteins
Neural Cell Adhesion Molecule L1