Diagnosis of tuberculosis based on the detection of a cocktail of mycobacterial antigen 85B, ESAT-6 and cord factor by immuno-PCR

J Microbiol Methods. 2016 Aug:127:24-27. doi: 10.1016/j.mimet.2016.05.003. Epub 2016 May 7.

Abstract

Attempts were made to enhance the sensitivity of immuno-PCR assay based on the detection of cocktail of mycobacterial antigen 85B (Rv1886c), ESAT-6 (Rv3875) and cord factor (trehalose 6,6'-dimycolate) in pulmonary and extrapulmonary TB patients. Detection of Ag85B was found to be superior to the detection of cocktail in TB patients.

Keywords: Antigen 85B; Cord factor; ESAT-6; Immuno-PCR; Tuberculosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / analysis*
  • Antigens, Bacterial / immunology
  • Cord Factors / analysis*
  • Cord Factors / immunology
  • Immunoassay / methods
  • Polymerase Chain Reaction / methods*
  • Tuberculosis / diagnosis*
  • Tuberculosis / microbiology
  • Tuberculosis, Pulmonary / diagnosis
  • Tuberculosis, Pulmonary / microbiology

Substances

  • Antigens, Bacterial
  • Cord Factors
  • ESAT-6 antigen, Mycobacterium leprae
  • antigen 85B, Mycobacterium leprae