New flow cytometry approaches in equine andrology

Fernando J Peña; Cristina Ortega Ferrusola; Patricia Martín Muñoz

doi:10.1016/j.theriogenology.2016.04.050

New flow cytometry approaches in equine andrology

Theriogenology. 2016 Jul 1;86(1):366-72. doi: 10.1016/j.theriogenology.2016.04.050. Epub 2016 Apr 21.

Authors

Fernando J Peña¹, Cristina Ortega Ferrusola², Patricia Martín Muñoz³

Affiliations

¹ University of Extremadura, Laboratory of Equine Reproduction and Spermatology, University of Extremadura, Cáceres, Spain. Electronic address: fjuanpvega@unex.es.
² Reproduction and Obstetrics, Department of Animal Medicine and Surgery, University of León, León, Spain.
³ University of Extremadura, Laboratory of Equine Reproduction and Spermatology, University of Extremadura, Cáceres, Spain.

PMID: 27160445
DOI: 10.1016/j.theriogenology.2016.04.050

Abstract

Flow cytometry is currently recognized as a robust tool for the evaluation of sperm quality and function. However, within equine reproduction, this technique has not reached the sophistication of other areas of biology and medicine. In recent years, more sophisticated flow cytometers have been introduced in andrology laboratories, and the number of tests that can be potentially used in the evaluation of sperm physiology has increased accordingly. In this review, recent advances in the evaluation of stallion spermatozoa will be discussed. These new techniques in flow cytometry are able to simultaneously measure damage to different sperm regions and/or changes in functionality.

Keywords: Multiparametric flow cytometry; Spermatozoon; Stallion.

Publication types

Review

MeSH terms

Animals
Flow Cytometry / methods
Flow Cytometry / veterinary*
Horses / physiology*
Male
Semen Analysis / veterinary*