Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold

Guoliang Ke; Minghui Liu; Shuoxing Jiang; Xiaodong Qi; Yuhe Renee Yang; Shaun Wootten; Fei Zhang; Zhi Zhu; Yan Liu; Chaoyong James Yang; Hao Yan

doi:10.1002/anie.201603183

Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold

Angew Chem Int Ed Engl. 2016 Jun 20;55(26):7483-6. doi: 10.1002/anie.201603183. Epub 2016 May 9.

Authors

Guoliang Ke^{1

2}, Minghui Liu¹, Shuoxing Jiang¹, Xiaodong Qi¹, Yuhe Renee Yang¹, Shaun Wootten¹, Fei Zhang¹, Zhi Zhu², Yan Liu³, Chaoyong James Yang⁴, Hao Yan⁵

Affiliations

¹ Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA.
² The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
³ Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA. hao.yan@asu.edu.
⁴ The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. cyyang@xmu.edu.cn.
⁵ Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA. yan_liu@asu.edu.

PMID: 27159899
DOI: 10.1002/anie.201603183

Abstract

Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH-MDH and G6pDH-LDH) through the control of NAD(+) substrate channeling by specifically shifting NAD(+) between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.

Keywords: DNA origami; NADH; enzymes; self-assembly; substrate channeling.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

DNA / chemistry*
Multienzyme Complexes / chemistry*
NAD / chemistry
Nanomedicine
Substrate Specificity
Synthetic Biology

Substances

Multienzyme Complexes
NAD
DNA