Assessment of biofilm production in Candida isolates according to species and origin of infection

Abstract

The biofilm production (BP) of 200 clinical strains of Candida isolated during 2010-2013 were assessed using an in vitro model and a comparison of the results was made between species and between origins of the infections. The BP was assessed using the crystal violet assay, and the strains were classified as low, moderate, or high biofilm producers. Candida tropicalis had the highest values for BP, which varied depending on the origin of the infection. Assessment of BP is a key diagnostic tool that enables us to better understand Candida infections.

Keywords: Biofilm production; Candida; Clinical samples; Crystal violet binding assay; Cut-off; Muestras clínicas; Producción de biopelícula; Punto de corte; Tinción cristal violeta.