Agrobacterium-mediated inoculation of chrysanthemum (Chrysanthemum morifolium) plants with chrysanthemum stunt viroid

Tomoyuki Nabeshima; Motoaki Doi; Munetaka Hosokawa

doi:10.1016/j.jviromet.2016.05.001

Agrobacterium-mediated inoculation of chrysanthemum (Chrysanthemum morifolium) plants with chrysanthemum stunt viroid

J Virol Methods. 2016 Aug:234:169-73. doi: 10.1016/j.jviromet.2016.05.001. Epub 2016 May 4.

Authors

Tomoyuki Nabeshima¹, Motoaki Doi², Munetaka Hosokawa³

Affiliations

¹ Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: nabeshima.tomoyuki.68w@st.kyoto-u.ac.jp.
² Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: dmotoaki@kais.kyoto-u.ac.jp.
³ Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: mune@kais.kyoto-u.ac.jp.

PMID: 27155239
DOI: 10.1016/j.jviromet.2016.05.001

Abstract

Agroinfiltration was tested as a method of inoculation of chrysanthemum plants with chrysanthemum stunt viroid (CSVd). Binary vectors harboring dimeric CSVd sequences in sense and antisense orientations were constructed, and Agrobacterium transfected with these binary vectors was infiltrated into chrysanthemum leaves. Northern blotting and reverse transcription polymerase chain reaction analysis showed that local infection was established within 7 days and systemic infection within 20 days. CSVd polarities showed no difference in infectivity. This study showed that agroinfiltration of chrysanthemum plants is an easy, rapid, and cost-effective method for CSVd inoculation.

Keywords: Agrobacterium; Chrysanthemum molifolium; Chrysanthemum stunt viroid; Infiltration; Viroid infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium / virology*
Chrysanthemum / microbiology*
Chrysanthemum / virology*
Plant Diseases / virology
Plant Leaves / virology
Viroids*