Increase in IS256 transposition in invasive vancomycin heteroresistant Staphylococcus aureus isolate belonging to ST100 and its derived VISA mutants

Sabrina Di Gregorio; Silvina Fernandez; Beatriz Perazzi; Natalia Bello; Angela Famiglietti; Marta Mollerach

doi:10.1016/j.meegid.2016.05.001

Increase in IS256 transposition in invasive vancomycin heteroresistant Staphylococcus aureus isolate belonging to ST100 and its derived VISA mutants

Infect Genet Evol. 2016 Sep:43:197-202. doi: 10.1016/j.meegid.2016.05.001. Epub 2016 May 3.

Authors

Sabrina Di Gregorio¹, Silvina Fernandez¹, Beatriz Perazzi², Natalia Bello³, Angela Famiglietti², Marta Mollerach⁴

Affiliations

¹ Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, CABA, Argentina.
² Laboratorio de Bacteriología Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, CABA, Argentina.
³ División Infectología, Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, CABA, Argentina.
⁴ Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, CABA, Argentina. Electronic address: martamollerach@gmail.com.

PMID: 27154328
DOI: 10.1016/j.meegid.2016.05.001

Abstract

In Staphylococcus aureus, transposition of IS256 has been described to play an important role in biofilm formation and antibiotic resistance. This study describes the molecular characterization of two clinical heterogeneous vancomycin-intermediate S. aureus (hVISA) isolates recovered from the same patient (before and after antibiotic treatment) and two VISA derivatives obtained by serial passages in the presence of vancomycin. Our results showed that antibiotic treatment (in vivo and in vitro) could enhance IS256 transposition, being responsible for the eventual loss of agr function. As far as we know this is the first study that reports the increase of IS256 transposition in isogenic strains after antibiotic treatment in a clinical setting.

Keywords: IS256 transposition; S. aureus; hVISA/VISA.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Bacterial Typing Techniques
Biofilms / drug effects*
Biofilms / growth & development
DNA Transposable Elements*
Gene Expression
Genotype
Humans
Male
Microbial Sensitivity Tests
Phosphoric Monoester Hydrolases / genetics*
Phosphoric Monoester Hydrolases / metabolism
Sigma Factor / genetics*
Sigma Factor / metabolism
Staphylococcal Infections / microbiology
Staphylococcus aureus / drug effects*
Staphylococcus aureus / genetics
Staphylococcus aureus / growth & development
Vancomycin / pharmacology
Vancomycin Resistance / genetics*

Substances

Anti-Bacterial Agents
Bacterial Proteins
DNA Transposable Elements
SigB protein, Bacteria
Sigma Factor
Vancomycin
Phosphoric Monoester Hydrolases