OpenSegSPIM: a user-friendly segmentation tool for SPIM data

Bioinformatics. 2016 Jul 1;32(13):2075-7. doi: 10.1093/bioinformatics/btw093. Epub 2016 Feb 22.

Abstract

OpenSegSPIM is an open access and user friendly 3D automatic quantitative analysis tool for Single Plane Illumination Microscopy data. The software is designed to extract, in a user-friendly way, quantitative relevant information from SPIM image stacks, such as the number of nuclei or cells. It provides quantitative measurement (volume, sphericity, distance, intensity) on Light Sheet Fluorescent Microscopy images.

Availability and implementation: freely available from http://www.opensegspim.weebly.com Source code and binaries under BSD License.

Contact: lgole@imcb.a-star.edu.sg or wmyu@imcb.a-star.edu.sg or sohail.ahmed@imb.a-star.edu.sg

Supplementary information: Supplementary data are available at Bioinformatics online.

MeSH terms

  • Algorithms
  • Animals
  • Cell Nucleus
  • Humans
  • Image Interpretation, Computer-Assisted*
  • Microscopy*
  • Software*