Screening for new antibiotics remains an important area of biology and medical science. Indispensable for this type of research is early identification of antibiotic mechanism of action. Preferentially, it should be studied quickly and cost-effectively, on the stage of primary screening. In this review we describe an application of reporter strains for rapid classification of antibiotics by its target, without prior purification of an active compound and determination of chemical structure.
Poisk novykh antibiotikov ostaetsia aktual'noĭ zadacheĭ biologii i meditsiny. Neobkhodimym étapom v issledovanii novykh antibiotikov iavliaetsia ustanovlenie mekhanizma ikh deĭstviia, prichem zhelatel'no bystro i deshevo, predpochtitel'no na étape pervichnogo skrininga. V predstavlennom obzore privedeny svedeniia o primenenii reporternykh shtammov dlia bystroĭ klassifikatsii antibiotikov po mekhanizmu deĭstviia, ne trebuiushcheĭ ochistki i opredeleniia stroeniia analiziruemykh soedineniĭ.
Keywords: antibiotic; bacteria; protein synthesis; reporter strain; screening.